Active Recombinant Canine IL17A protein, His-tagged
Cat.No. : | IL17A-1588C |
Product Overview : | Recombinant Canine IL17A protein(Gly26-Ala155), fused with His tag, was expressed in HEK293. |
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Species : | Canine |
Source : | HEK293 |
Tag : | His |
Protein Length : | 26-155 a.a. |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
Bio-activity : | Immobilized Monoclonal Anti-Human IL17A Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine IL-17A, His Tag with a linear range of 5-39 ng/mL.Immobilized Canine IL-17A, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag with a linear range of 4-63 ng/mL.Immobilized Canine IL-17A, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) with a linear range of 0.1-4 ng/mL. |
Molecular Mass : | This protein carries a polyhistidine tag at the N-terminusThe protein has a calculated MW of 17.0 kDa. The protein migrates as 18 kDa and 22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : | Gly26-Ala155 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Storage : | For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
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Not For Human Consumption!
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