Active Recombinant Canine EFNA5 protein, Fc-tagged

• Cat.No.: EFNA5-993C
• Product Overview: Recombinant Canine Efna5(Met1-Asn203) fused with Fc region of human IgG1 tag at C-terminal was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Asn203
• Predicted N Terminal: Gln 21
• Form: Lyophilized from sterile PBS, pH 7.4.1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Bio-activity: Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind canine EFNA5-Fc, The EC50 of canine EFNA5-Fc is 11.5-26.7 ng/ml.
• Molecular Mass: The recombinant canine EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 424 amino acids and has a predicted molecular mass of 48.2 kDa. The apparent molecular mass of the protein is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: LOC608458 ephrin-A5-like [ Canis lupus familiaris ]
• Official Symbol: EFNA5
• Synonyms: EFNA5; ephrin-A5
• Gene ID: 608458
• mRNA Refseq: XM_845489
• Protein Refseq: XP_850582
• Chromosome Location: chromosome: 3
• Pathway: Axon guidance; PI3K-Akt signaling pathway