Active Recombinant Cynomolgus EFNA5 protein, Fc-tagged

• Cat.No.: EFNA5-1431C
• Product Overview: Recombinant Cynomolgus Efna5(Met1-Asn203) fused with Fc region of human IgG1 tag at C-terminal was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Asn203
• Predicted N Terminal: Gln 21
• Form: Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Cynomolgus EFNA5-Fc at 10 μg/mL (100 μl/well) can bind biotinylated Cynomolgus EPHA4. The EC50 of biotinylated Cynomolgus EPHA4 is 9.3-21.6 ng/mL.
• Molecular Mass: The recombinant cynomolgus EFNA5/Fc is a disulfide-linked homodimer The reduced monomer consists of 424 amino acids and has a calculated molecular mass of 48.2 kDa. The apparent molecular mass of the reduced cynoEFNA5/Fc monomer is approximately 48 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: EFNA5 ephrin-A5 [ Macaca fascicularis ]
• Official Symbol: EFNA5
• Synonyms: ephrin-A5; EPH-related receptor tyrosine kinase ligand 7
• Gene ID: 102141609
• Chromosome Location: chromosome: 6
• Pathway: Axon guidance, organism-specific biosystem; PI3K-Akt signaling pathway, organism-specific biosystem; Rap1 signaling pathway, conserved biosystem