Active Recombinant Canine Chemokine (C-C motif) Ligand 2

• Cat.No.: CCL2-406C
• Product Overview: Recombinant Canine CCL2 protein wasexpressed in E. coli. Gln24-Pro101.

Specification

• Species: Canine
• Source: E.coli
• Tag: Non
• Protein Length: 24-101 a.a.
• Description: Canine CCL2is an 8 kDa member of the CC chemokine family of chemotactic factors. It issynthesized as a 101 amino acid (aa) precursor that contains a 23 aa signalsequence and a 78 aa mature segment. It contains no potential N-linked glycosylationsites and is not known for any posttranslational modifications.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminal Sequence: Noresults obtained: Gln24 predicted
• Molecular Weight: 8.8 kDa
• Activity: Measured by itsability to chemo-attract human CCR2A transected BaF3 Canine pro-B cells. TheED50 for this effect is typically 0.03-0.15 μg/mL.
• Endotoxin Level: <1.0 EU per 1μgof the protein by the LAL method.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 25 μg/mL insterile PBS that containing at least 0.1% human or bovine serum albumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month,2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: CCL2 chemokine (C-C motif)ligand 2 [ Canis lupus familiaris ]
• Official Symbol: CCL2
• Synonyms: CCL2; chemokine(C-C motif) ligand 2; C-C motif chemokin 2; MCP-1; small-inducible cytokineA2; momocyte chemo attractant protein 1; momocyte chemo attractant protein-1;MCP1; SCYA2
• Gene ID: 403981
• mRNA Refseq: NM_001003297
• Protein Refseq: NP_001003297
• UniProt ID: P52203
• Chromosome Location: 9
• Pathway: Chagasdisease (American trypanosomiasis); Chemokine signaling pathway; Cytokine-cytokinereceptor interaction; Diabetes pathways; Herpes simplex infection;Influenza A; Malaria; NOD-like receptor signaling pathway; PERK regulatedgene expression; Rheumatoid arthritis; Unfolded Protein Response