Active Recombinant Canine CA9 Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CA9-1544CAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant canine CA9 (Met1-Leu410) was expressed in human HEK 293 cells and fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Dog
• Source: HEK293
• Tag: Fc
• ProteinLength: 614
• Form: Lyophilized Powder
• Bio-activity: Measured by its esterase activity. The specific activity is > 10 pmoles/min/ μg.
• Molecular Mass: The recombinant canine CA9/Fc is a disulfide-linked homodimer. The reduced monomer comprises 614 amino acids and has a predicted molecular mass of 67.6 kDa. The apparent molecular mass of the protein is approximately 79 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU/ μg protein as determined by the LAL method.
• Purity: > 99 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: 25 mM Tris, 0.15 M NaCl, pH 7.5

Gene Information

• Gene Name: CA9 carbonic anhydrase 9 [ Canis lupus familiaris (dog) ]
• Official Symbol: CA9
• Synonyms: CA9; carbonic anhydrase 9
• Gene ID: 611933
• mRNA Refseq: NM_001145174
• Protein Refseq: NP_001138646
• UniProt ID: E2RAI4