Active Recombinant Canine Advanced Glycosylation End Product-Specific Receptor, His-tagged

• Cat.No.: AGER-537C
• Product Overview: Recombinant Canine AGERprotein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Asp25-Val339.

Specification

• Cat. No.: AGER-537C
• Description: Advanced glycationendproducts (AGEs) are adducts formed by the nonenzymatic glycation of macromolecules.AGE formation is accelerated in oxidative and hyperglycemic conditions,diabetes, renal failure, atherosclerosis, Alzheimer's disease, arthritis, andin normal aging. Receptor for advanced glycation endproducts (RAGE) is a 35kDa type I transmembrane protein belonging the immunoglobulin superfamily.Besides AGEs, RAGE binds βamyloid peptide, S100/calgranulin family proteins,HMGB1/amphoterin, and leukocyte integrins. Mature canine RAGE consists of a 383amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain andtwo Ig-like C-type domains, a 23 aa transmembrane segment, and a 43 aacytoplasmic domain.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Asp25
• Molecular Weight: 34.6 kDa
• Activity: Measured by itsability to bind biotinylated advanced glycation endproducts of bovine serumalbumin.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 40-44 kDa, reducingconditions.
• Purity: > 90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 25-339 a.a.

Gene Information

• Gene Name: AGER advanced glycosylationend product-specific receptor [ Canis lupus familiaris ]
• Official Symbol: AGER
• Synonyms: AGER; advancedglycosylation end product-specific receptor; RAGE; RAGE/AGER; advancedglycation end product receptor
• Gene ID: 403168
• mRNA Refseq: NM_001048081
• Protein Refseq: NP_001041546
• Chromosome Location: 12
• Pathway: Activated TLR4signalling; Immune System; Innate Immune System; MyD88 cascade initiated onplasma membrane; MyD88 dependent cascade initiated on endosome; MyD88-independentcascade initiated on plasma membrane; MyD88:Mal cascade initiated on plasmamembrane; NFkB and MAP kinases activation mediated by TLR4 signalingrepertoire; RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways; TAK1activates NFkB by phosphorylation and activation of IKKs complex; TRAF6Mediated Induction of proinflammatory cytokines; TRAF6 mediated NF-kBactivation; TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9activation; TRIF mediated TLR3 signaling; Toll Like Receptor 10 (TLR10)Cascade