Recombinant Human AGER Protein (Met1-Ala342), HlgG1 Fc-tagged

• Cat.No.: AGER-1061H
• Product Overview: Recombinant Human AGER Protein (NP_001127.1) (Met1-Ala342) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Description: Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S1/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). Initial studies investigating the role of RAGE in renal dysfunction focused on diabetes, neurodegenerative disorders, and inflammatory responses. However, RAGE also has roles in the pathogenesis of renal disorders that are not associated with diabetes, such as obesity-related glomerulopathy, doxorubicin-induced nephropathy, hypertensive nephropathy, lupus nephritis, renal amyloidosis, and ischemic renal injuries. RAGE represents an important factor in innate immunity against pathogens, but it also interacts with endogenous ligands, resulting in chronic inflammation. RAGE signaling has been implicated in multiple human illnesses, including atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
• Source: HEK293
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ala 23
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human AGER consists of 553 amino acids and predicts a molecular mass of 60.1 kDa.
• Protein length: Met1-Ala342
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: AGER advanced glycosylation end product-specific receptor [ Homo sapiens ]
• Official Symbol: AGER
• Synonyms: AGER; RAGE; RAGE isoform sRAGE-delta
• Gene ID: 177
• mRNA Refseq: NM_001136
• Protein Refseq: NP_001127
• MIM: 600214
• UniProt ID: Q15109