Active Recombinant Canine ACVRL1 protein, hFc-tagged

• Cat.No.: ACVRL1-318C
• Product Overview: Recombinant Canine ACVRL1 protein(E2R174)(Met1-Gln119), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Gln119
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombinant human BMP-9.
• Molecular Mass: The recombinant canine ACVRL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 339 amino acids and has a predicted molecular mass of 37.9 kDa. The apparent molecular mass of the protein is approximately 43-47 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ACVRL1 activin A receptor type II-like 1 [ Canis lupus familiaris (dog) ]
• Official Symbol: ACVRL1
• Synonyms: ACVRL1; activin A receptor type II-like 1; serine/threonine-protein kinase receptor R3; activin A receptor type II-like 1
• Gene ID: 477603
• mRNA Refseq: XM_534796
• Protein Refseq: XP_534796