Active Recombinant Bovine Interleukin 13

• Cat.No.: IL13-234B
• Product Overview: Recombinant Bovine IL-13 was expressed inYeast. MW= 12.6 kDa (112 aa).

Specification

• Species: Bovine
• Source: Yeast
• Tag: Non
• Description: IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
• Formulation/ Reconstitution: Lyophilized. Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Purification: Ion-exchange chromatography.
• Purity: >95% as visualized by SDS-PAGE analysis.
• Amino Acid Sequence: SPVPSATALK ELIEELVNIT QNQKVPLCNG SMVWSLNLTS SMYCAALDSL ISISNCSVIQ RTKRMLNALC PHKPSAKQVS SEYVRDTKIE VAQFLKDLLR HSRIVFRNER FN.
• Bioactivity: The biological activity of recombinant bovine IL-13 was measured in a cell proliferation assay using the human TF-1 cell line. The ED50 for this effect is typically 20 - 30 ng/mL.
• Stability And Storage: Stable for up to twelve months from date of receipt at -20°C. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: IL13 interleukin 13 [ Bos taurus ]
• Synonyms: interleukin 13; IL13; interleukin-13; IL-13
• Gene ID: 281247
• mRNA Refseq: NM_174089.1
• Protein Refseq: NP_776514.1
• UniProt ID: Q9XSV9
• Chromosome Location: 7
• Pathway: Asthma; Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Jak-STAT signaling pathway
• Function: cytokine activity; interleukin-13 receptor binding; protein binding