| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
N-hFc |
| Protein length : |
Gly21-Asn132 |
| Form : |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 ug/ml (100 ul/well) on the streptavidin precoated plate (5 ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8 ng/ml determined by ELISA (QC Test). |
| Molecular Mass : |
The protein has a predicted MW of 39.6 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Endotoxin : |
Less than 1EU per μg by the LAL method. |
| Purity : |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
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