Active Recombinant Bovine DNase I

• Cat.No.: DNase I-04B
• Product Overview: Recombinant DNase I is derived from bovine pancreas and recombinantly expressed in Pichia pastoris. The RNase activity has been removed during the purification process. RNase Activity: Negative Residual Host Cell Protein: < 50 ppm Residual Host Cell DNA: < 100 pg/mg pH: 7.8-8.2

Specification

• Species: Bovine
• Source: Pichia
• Tag: Non
• Description: DNase I is a non-specific endonuclease that can degrade double-stranded and single-stranded DNA as well as chromosomes. It acts by hydrolyzing phosphodiester bonds to produce mononucleotides and oligonucleotides with 5'-phosphate groups and 3'-hydroxyl groups. The activity of this enzyme requires the participation of Mg2+ or Ca2+ ions, and the optimal pH is approximately 7.8.
• Purity: ≥ 95% by SDS-PAGE
• Bio-activity: ≥ 2 U/μL
• Advantages: 1. RNase-free. 2. It has high purity and can be directly used for amplification reactions. 3. Its performance is comparable to that of well-known brand.
• Unit definition: 1 U is defined as the amount of enzyme required to completely degrade 1 μg of plasmid DNA at 37 centigrade within 10 minutes.
• Endotoxin: < 10 EU/mg
• Usage: RNA: 10 μg 10× DNase I Reaction Buffer: 10 μL Recombinant DNase I: 2 U Water: Up to 100 μL Incubate at 37 centigrade for 10 minutes. After the reaction is completed, add EDTA to a final concentration of 5 mM and incubate at 75 centigrade for 10 minutes to inactivate DNase I.
• Applications: 1. Removal of template DNA after in vitro transcription 2. Removal of DNA contamination in RNA
• Note: 1. Try to avoid freeze-thaw cycles of this product after receipt; 2. Please wear lab coat and disposable gloves when using; 3. Inactivation: Add EDTA to a final concentration of 5 mM and heat at 75 centigrade for 10 minutes to inactivate DNase I; 4. This product should not be used directly for clinical diagnosis and treatment.
• Stability: This product should be stored at -20 centigrade and can be stored for at least 24 months.
• Storage: Store at -20 centigrade.
• Shipping: Dry Ice

Gene Information

• Official Symbol: DNase I