Active Recombinant Bovine Betacellulin

• Cat.No.: BTC-320B
• Product Overview: Recombinant Bovine Betacellulin was produced inE.Coli.

Specification

• Species: Bovine
• Source: E.coli
• Tag: Non
• Description: Bovine Betacellulin (BTC), a member of the EGF family, is an 80 amino acid heparin-binding protein. Like other members of the EGF family, it is synthesized as a transmembrane precursor. The soluble cytokine, containing one EGF structural motif, is released by proteolytic cleavage. BTC can bind to the EGF receptor (ErbB-1) which then dimerizes with another member of the ErbB receptor family to signal through the tyrosine kinase pathway. BTC is a potent mitogen for Balb/c 3T3 fibroblasts, retinal pigment epithelial cells and vascular smooth muscle cells and is the only member of the EGF family to stimulate insulin secretion in pancreatic b cells.
• Purity: By N-terminal sequencing: >95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC.
• Molecular Weight: Theoretical: 8995 daltons. By Mass Spectrometry the product is 50% [Gly1]BTC (9052 daltons) and 50% Des(1-4)BTC (8709 daltons).
• N-terminal Sequence Analysis: 5 residues >95% bovine betacellulin, comprising an equal mixture of [Gly1]BTC and Des(1-4)BTC.
• Activity: Cell growth bioassay in Balb/c 3T3 fibroblasts: ED₅₀<10 ng/ml.
• Endotoxin: <0.1 EU/µg.
• State And Appearance: Lyophilized white powder. Dried from 0.05 M acetic acid, 0.05% (v/v) trifluoroacetic acid and stored under dry nitrogen at a slight vacuum (-25 kPa).
• Storage: At least 2 years at 2 – 4°C(lyophilized).

Gene Information

• Gene Name: BTC betacellulin [ Bos taurus ]
• Synonyms: Betacellulin; BTC
• Gene ID: 280737
• mRNA Refseq: NM_173896
• Protein Refseq: NP_776321
• UniProt ID: Q9TTC5
• Chromosome Location: 6
• Pathway: ErbB signaling pathway
• Function: Growth factor activity