Active Recombinant Bacteriophage Lambda Exonuclease

• Cat.No.: xis-56B
• Product Overview: Purified from a strain of E. coli that overexpresses the exonuclease gene from bacteriophage Lambda.

Specification

• Species: Bacteriophage Lambda
• Source: E.coli
• Tag: Non
• Description: Lambda Exonuclease is a highly processive 5′→3′ double-stranded exonuclease that degrades one strand of the duplex. Lambda exonuclease can initiate at blunt DNA or DNA containing 3′ single-stranded overhangs. Lambda Exonuclease has greatly reduced activity on non-phosphorylated DNA, single-stranded DNA and DNA having protruding 5′ single-stranded termini. Lambda exonuclease will not initiate at a nick or gap.
• Bio-activity: 80,000 U/mg
• Molecular Mass: 25,909 Daltons
• Purity: >99% by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in 30 minutes at 37°C.
• Storage: -25 to -15°C
• Concentration: 5,000 U/mL
• Storage Buffer: Supplied in: 25mM Tris-HCl, 50mM NaCl, 1mM DTT, 0.1mM EDTA, 50% glycerol pH 7.5 @ 25?C. Supplied with: 10X Lambda Exo Reaction Buffer: 670 mM Glycine, 25mM MgCl2, 10mM DTT, pH 9.4 @25°C

Gene Information

• Gene Name: xis exonuclease [ Enterobacteria phage lambda ]
• Official Symbol: xis
• Synonyms: xis; exonuclease; NP_040616.1
• Gene ID: 2703469
• Protein Refseq: NP_040616
• UniProt ID: 0902231Q