Active Native Spindle tree Euonymus europaeus Lectin (EEL/EEA), AP-Conjugated

• Cat.No.: EEL/EEA-011S
• Product Overview: Native Spindle tree Euonymus europaeus Lectin (EEL/EEA) - AP (Alkaline Phosphatase)
  Blood Group Specificity: Non-specific
  Preferred Sugar Specificity: Galα3Gal
  Inhibiting or Eluting Sugar: Lactose
  Divalent Ions: Ca++, Zn++
  Mitogenic Activity: Yes
  Hazardous Shipping: Non-hazardous
  Carbohydrate Specificity: Galα3Gal, high mannose N-type glycans

Specification

• Description: Euonymus europaeus lectin or agglutinin (EEL/EEA) is affinity purified and is a homodimer consisting of subunits of 17 kDa linked by disulfide bonds. EEL consists of six closely related lectins (isoforms). Previous reports of higher molecular weight and overestimated carbohydrate percentage are attributed to lectin-glycoprotein complexes and not the Euonymus europaeus lectin itself. This lectin is characteristic of its erythrocyte agglutinating properties and specificity for blood groups B and H. It has a stronger binding of the B oligosaccharide with higher affinity for B-type II in comparison to H structures with Galα1,3 structures. Conversely, it has also been reported that EEL has preferential binding to H blood group-related carbohydrates over the B bloof group due to similarities in the β-trefoil fold found in EEL and ricin B-like lectins. EEL’s general pattern allows it to interact with both α-fucose residues on B and H blood groups as well as high mannose N-type glycans. This lectin binds to endothelial cells from human and non-human sources and recognizes carbohydrate structures on the surface of stimulated murine peritoneal lymphoid cells. EEL also has applications as a endothelial/epithelial marker in canine tissues and has been studied in Kashin-Beck disease. Alkaline phosphatase (AP) is conjugated to WGA to show the binding of WGA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
• Source: Spindle tree
• Species: Spindle tree
• Form: Liquid
• Bio-activity: 50 mg/ml will agglutinate human A2 erythrocytes.
• Purity: High Purity Grade
• Stability: Shelf Life: 1 year
• Storage: Store frozen at -20 centigrade in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 centigrade for short term use. Clarify by centrifugation, if needed.
• Concentration: 1 mg/mL

Gene Information

• Official Symbol: EEL/EEA
• Synonyms: Euonymus europaeus Lectin; EEL/EEA