Active Native Flavobacterium heparinum 2-O-Sulfatase

• Cat.No.: SULF2-02F

Specification

• Description: Hydrolyses the terminal 2-O-sulphate on the unsaturated di- and oligo-saccharides produced by the action of lyases on sulphated glycosaminoglycans.
• Form: Solution contains 10 mM tris-acetate, pH 7.3, 80 mM NaCl, 10 mM potassium phosphate, 0.2% BSA.
• Bio-activity: 10-40 (units/ml)
• Protein length: 843
• Enzyme Unit: One unit will hydrolyze heparin disaccharide I-P (H9401) to yield 1.0 micromole of disaccharide II-P (δ-UA-GlcNCOEt-6S) per minute at pH 7.3 at 25 °C.
• Applications: 2-O-Sulfatase from Flavobacterium heparinum has been used in a study to determine that oversulfated chondroitin sulfate is a contaminant in heparin associated with adverse clinical events.2-O-Sulfatase from Flavobacterium heparinum has also been used in a study to determine the structure of oligosaccharides prepared from acharan sulfate.
• Storage: at -70 °C
• Tag: Non