Active Native Clostridium difficile Toxin A Protein

• Cat.No.: TcdA-188C
• Product Overview: Native Toxin A purified from Clostridium difficile.

Specification

• Species: Clostridium difficile
• Source: Clostridium difficile
• Description: Toxin A from Clostridium difficile, also known as TcdA, is an enterotoxin that has glucosyltransferase and cysteine protease activities. It inactivates host Rho family GTPases by glucosylation, altering cytoskeletal structure, cell division, and cell-cell adhesion. Toxin A can induce activation of the inflammasome and apoptosis. While both toxin A and toxin B inactivate Rho family GTPases, toxin A has a higher binding affinity for epithelial cell surface carbohydrates.
• Bio-activity: EC50 of < 0.2 ng/mL, as tested on Vero cells.
• Purity: ≥80% estimated by SDS-PAGE
• Stability: ≥ 2 years
• Storage: At 4 centigrade.
• Storage Buffer: 50 mM Tris, 50 mM NaCl, pH 7.5, and 0.1% trahalose
• Reconstitution: 0.5 mL of water

Gene Information

• Synonyms: TcdA; Toxin A
• UniProt ID: P16154