Active Native Bordetella bronchiseptica Dermonecrotic Toxin

• Cat.No.: dnt-142B
• Product Overview: DNT was purified from B. bronchiseptica strain S798 by propriety chromatography and differential precipitation method.

Specification

• Species: B.bronchiseptica
• Source: B.bronchiseptica
• Tag: Non
• Description: Dermonecrotic toxin (DNT) is a common toxin produced by pathogenic bacteria belonging to the genus Bordetella, such as B. pertussis, B. parapertussis, and B. bronchiseptica. This toxin activates small GTP-binding proteins such as Rho, Rac and Cdc42 through deamidation or polyamination for downstream signaling pathways.
• Form: 0.53 mg/ml of DNT in 50 mM sodium phosphate buffer (pH 7.2), 1 M urea, 0.3 M sodium sulfate, filter-sterilized.
• Bio-activity: DNT at 5 ng/ml induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.
• Purity: More than 95 % purity by SDS-PAGE (CBB staining)
• Applications: 1) For the studies of the signaling pathways mediated by small GTP-binding proteins. 2) Antigen to be used for detection of infection of Bordetella by immunological method.
• Storage: Store at 4℃