Active Native Bacillus stearothermophilus Acetate Kinase

• Cat.No.: Acetate Kinase-22B
• Product Overview: Native Bacillus stearothermophilus Acetate Kinase was purified from Bacillus stearothermophilus.

Specification

• Description: Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 α-helix and 43 % unordered structure.
• Source: Bacillus stearothermophilus
• Species: Bacillus stearothermophilus
• Form: Lyophilized powder containing potassium phosphate.
• Bio-activity: Involved in the metabolism of propanoate, pyruvate and taurine. 400-1,200 units/mg solid.
• Unit Definition: One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.2 at 30 °C.
• Applications: Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
• Storage: 2-8°C.
• Tag: Non