Active GMP Recombinant Human IL4 Protein

• Cat.No.: IL4-01HG
• Product Overview: Recombinant human IL4 protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Description: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. IL4 is considered an important cytokine for tissue repair, counterbalancing the effects of proinflammatory type 1 cytokines, however, it also promotes allergic airway inflammation. Moreover, IL-4, a type 2 cytokine, mediates and regulates a variety of human host responses such as allergic, anti-parasitic, wound healing, and acute inflammation. This cytokine has been reported to promote resolution of neutrophil-mediated acute lung injury. In an allergic response, IL-4 has an essential role in the production of allergen-specific immunoglobin (Ig) E. This pro-inflammatory cytokine has been observed to be increased in COVID-19 (Coronavirus disease 2019) patients, but is not necessarily associated with severe COVID-19 pathology. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Form: Lyophilized powder
• Endotoxin: <20 EU/mg
• Bio-activity: >5.0 x 10^6 IU/mg
• Purity: >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE.
• Storage Buffer: 10mM Phosphate-Buffered Saline (pH 7.2)
• Storage: Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing.
• Sterility: Sterility
• Instructions: It is recommended that lyophilized GMP rhIL4 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration.

Gene Information

• Gene Name: IL4 interleukin 4 [ Homo sapiens (human) ]
• Official Symbol: IL4
• Synonyms: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Gene ID: 3565
• mRNA Refseq: NM_000589.4
• Protein Refseq: NP_000580.1
• UniProt ID: P05112
• MIM: 47780