Active GMP Recombinant Human EGF Protein

• Cat.No.: EGF-830HG
• Product Overview: Recombinant mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified with an initial Met at the N-terminus in an animal component free process under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80.
• Bio-activity: Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.1-0.5 ng/mL. The specific activity of Recombinant Human EGF is approximately 1.9×10^3 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
• Molecular Mass: The GMP recombinant human EGF consists of 54 amino acids and has a calculated molecular mass of 6.3 kDa.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Protein length: 971-1023 a.a.

Gene Information

• Gene Name: EGF epidermal growth factor [ Homo sapiens ]
• Official Symbol: EGF
• Synonyms: EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4
• Gene ID: 1950
• mRNA Refseq: NM_001178130
• Protein Refseq: NP_001171601
• MIM: 131530
• UniProt ID: P01133