Active GMP Recombinant Human CD40LG protein

• Cat.No.: CD40LG-765HG
• Product Overview: GMP Recombinant Human CD40LG protein(NP_000065)(149 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Protein Length: DNA sequence encoding Human CD40 Ligand(UniProtKB/Swiss-Prot: P29965.1) was expressed with His tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The concentration of immobilized Human CD40 Ligand (His tag) is 2ug/mL(100uL/well), and the linear binding range that can bind to Human CD40(Fc tag) is 0.2-2.5 ug/mL.
• Molecular Mass: Recombinant Human CD40 protein contains 149 aa. It has a predicted MW of 18.5 kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
• Official Symbol: CD40LG
• Synonyms: CD40LG; CD154; CD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP; gp39
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965