Active GMP Recombinant Human CD40LG protein
Cat.No. : | CD40LG-4349HG |
Product Overview : | Recombinant Human CD40LG was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines. |
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Species : | Human |
Source : | E.coli |
Tag : | Non |
Description : | CD40 ligand is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family and can be cleaved into two chains: the CD40 ligand membrane form and CD40 ligand soluble form. It is primarily expressed on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. It mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation. |
Form : | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml. |
Molecular Mass : | Approximately 16.3 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids. |
AA Sequence : | MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL |
Endotoxin : | Less than 1 EU/μg of rHusCD40L as determined by LAL method. |
Purity : | > 95 % by SDS-PAGE and HPLC analyses. |
Storage : | This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably des centigradecated. Upon reconsitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw centigrade centigradeles. |
Reconstitution : | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Gene Name | CD40LG CD40 ligand [ Homo sapiens ] |
Official Symbol | CD40LG |
Synonyms | CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5; |
Gene ID | 959 |
mRNA Refseq | NM_000074 |
Protein Refseq | NP_000065 |
MIM | 300386 |
UniProt ID | P29965 |
◆ Recombinant Proteins | ||
CD40LG-784H | Recombinant Human CD40LG protein, His-tagged | +Inquiry |
CD40LG-166C | Recombinant Canine CD40LG | +Inquiry |
Cd40lg-7854M | Recombinant Mouse Cd40lg protein, monomeric hFc-tagged | +Inquiry |
CD40LG-167C | Recombinant Canine CD40LG, Fc-tagged | +Inquiry |
Cd40lg-2063M | Active Recombinant Mouse Cd40lg Protein | +Inquiry |
◆ Cell & Tissue Lysates | ||
CD40LG-1080CCL | Recombinant Cynomolgus CD40LG cell lysate | +Inquiry |
CD40LG-001CCL | Recombinant Canine CD40LG cell lysate | +Inquiry |
CD40LG-1965HCL | Recombinant Human CD40LG cell lysate | +Inquiry |
CD40LG-1548MCL | Recombinant Mouse CD40LG cell lysate | +Inquiry |
CD40LG-1205RCL | Recombinant Rat CD40LG cell lysate | +Inquiry |
Not For Human Consumption!
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