Active GMP Recombinant Human CD28 protein, Fc-tagged

• Cat.No.: CD28-332HG
• Product Overview: GMP Recombinant Human CD28 protein(NP_001230006)(366 aa), fused with Fc Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: DNA sequence encoding Human CD28(GenBank: AAH93698.1) was expressed with Fc tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The linear range of immobilized Human CD28 (Fc Tag) at 1 ug/mL(100 uL/well) that can bind to Anti-mouse CD28 antibody is 0.5-5 ng/mL.
• Molecular Mass: Recombinant Human CD28 protein contains 366 aa. It has a predicted MW of 42.1 kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; Tp44
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747