Active CD47 protein-coupled magnetic Beads

• Cat.No.: CD47-0989M

Specification

• Species: Human
• Source: HEK293
• Protein Length: Gln 19 - Pro 139
• Appearance: Powder mixture
• Particle size: 2 μm
• Bio-activity: Immobilized 15.8 μg CD47/1mg beads can bind the Anti CD47 Ab with an EC50 of 0.5720 μg/mL.
• Coupled amount of protein: ≈1302 pmol (22 μg) CD47 protein/mg beads
• Capacity: >250 pmol (45 μg) Anti-CD47 antibody/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The Human CD47-coupled Magnetic Beads is produced by coupling biotinylated CD47 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD47 antibody from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.