Acitve Recombinant Mouse/Rat VEGFC protein(Ala108-Arg223), Fc-tagged

• Cat.No.: VEGFC-1319M
• Product Overview: Recombinant Mouse / Rat VEGFC (NP_033532.1/O35757) (Ala108-Arg223) was expressed in HEK293, fused with the Fc region of human IgG1 at the N-terminus. Mouse and Rat mature VEGFC sequences are identical.

Specification

• Species: Mouse/Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Ala108-Arg223
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Mouse FLT4-His at 10 μg/mL (100 μL/well) can bind Mouse/Rat Fc-VEGFC, The EC50 of Mouse/Rat Fc-VEGFC is 0.41-0.95 μg/mL. 2. Measured in a cell prolifeRation assay using Human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 70-300 ng/mL.
• Molecular Mass: The recombinant Mouse / Rat VEGFC/Fc comprises 376 amino acids and has a predicted molecular mass of 41.5 kDa. The apparent molecular mass of the protein is approximately 44 and 34 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Vegfc vascular endothelial growth factor C [ Mus musculus ]
• Official Symbol: VEGFC
• Synonyms: VEGFC; vascular endothelial growth factor C; VRP; flt4-L; flt4 ligand; vascular endothelial growth factor C isoform 129; vascular endothelial growth factor C isoform 184; vascular endothelial growth factor-related protein; VEGF-C; AW228853
• Gene ID: 22341
• mRNA Refseq: NM_009506
• Protein Refseq: NP_033532