Source : |
HEK293 |
Species : |
Human |
Tag : |
His |
Protein length : |
Met1-Ser328 |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. |
Molecular Mass : |
The recombinant human IL12B consists of 317 amino acids and has a predicted molecular mass of 36.2 kDa. As a result of glycosylation, the apparent molecular mass of rhIL12B is approximately 40-50 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 95 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |