Acitve Recombinant Human SPHK1 protein(Met1-Leu384)

• Cat.No.: SPHK1-1331H
• Product Overview: Recombinant Human SPHK1 (Q9NYA1-1)(Met1-Leu384) was expressed in Insect Cells, fused with two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: Non
• Protein length: Met1-Leu384
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
• Bio-activity: The specific activity was determined to be ＞500nmol/min/mg using Sphingosine Kinase SubstRate as substRate.
• Molecular Mass: The recombinant Human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migRates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Official Symbol: SPHK1
• Synonyms: SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1