Recombinant Mouse Leptin

• Cat.No.: Lep-6933M
• Product Overview: Recombinant mouse Lep was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 16 kDa.

Specification

• Description: Lep is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility.
• Source: E. coli
• Species: Mouse
• Tag: Non
• Form: Liquid. In Phosphate buffered saline (pH 7.4).
• Molecular Mass: 16 kDa (147 aa), confirmed by MALDI-TOF.
• Protein length: 1-250aa
• AA Sequence: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Lep leptin [ Mus musculus ]
• Official Symbol: Lep
• Synonyms: LEP; leptin; obesity factor; ob; obese
• Gene ID: 16846
• mRNA Refseq: NM_008493
• Protein Refseq: NP_032519
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem
• Function: growth factor activity; hormone activity; peptide hormone receptor binding; protein binding