Acitve Recombinant Human IL12B&IL23A protein(Met1-Ser328 & Arg20-Pro189), His&Avi-tagged, Biotinylated

• Cat.No.: IL12B&IL23A-1379H
• Product Overview: Biotinylated Recombinant Human IL23 complex composed of Human IL12B (NP_002178.2) (Met1-Ser328) and Human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was expressed in HEK293, fused with a c-terminal AVI tagged polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met1-Ser328 & Arg20-Pro189
• Conjugation/Label: Biotin
• Form: Supplied as sterile 50 mM Tris, 100 mM Nacl, 10%glycerol, 0.02% Tween 80, 10% Trehalose, pH 8.5.
• Bio-activity: Immobilized Human IL23 Receptor Protein (ECD, hFc Tag) at 2 μg/mL (100 μL/well) can bind Human IL-23 Protein (His & AVI Tag), Biotinylated, the EC50 is 80-480ng/mL.
• Molecular Mass: The recombinant Human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol: IL12B; IL23A
• Synonyms: IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460