Acitve Recombinant Human EFNB1 protein(Met 1-Lys 237), His&hFc-tagged

• Cat.No.: EFNB1-1375H
• Product Overview: Recombinant Human EFNB1 (NP_004420.1)(Met 1-Lys 237) was expressed in HEK293, fused with a C-terminal Fc region of human IgG1 tag followed by a polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met 1-Lys 237
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB3 at 2 μg/ml (100 μl/well) can bind Human EFNB1 Fc chimera with a linear range of 1.56-25 ng/ml.
• Molecular Mass: The recombinant Human EFNB1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 64 and 36 KDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EFNB1 ephrin-B1 [ Homo sapiens ]
• Official Symbol: EFNB1
• Synonyms: EFNB1; ephrin-B1; CFNS, craniofrontonasal syndrome (craniofrontonasal dysplasia) , EPLG2; Elk L; LERK2; EFL-3; LERK-2; ELK ligand; ligand of eph-related kinase 2; eph-related receptor tyrosine kinase ligand 2; CFND; CFNS; EFL3; EPLG2; Elk-L; MGC8782
• Gene ID: 1947
• mRNA Refseq: NM_004429
• Protein Refseq: NP_004420
• MIM: 300035
• UniProt ID: P98172