Acitve Recombinant Human CD80 protein(Met1-Asn242), His&Avi-tagged, Biotinylated

• Cat.No.: CD80-1376H
• Product Overview: Biotinylated Recombinant Human CD80 (NP_005182.1)(Met1-Asn242) was expressed in HEK293 with a C-terminal polyhistidine tag followed by an AVI tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met1-Asn242
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CTLA-4 hFc at 2 μg/mL (100 μL/well) can bind Human B7-1, Biotinylated, the EC50 of Human B7-1, Biotinylated is 60-320 ng/mL.
• Molecular Mass: The recombinant Human CD80 consists of 234 amino acids and predicts a molecular mass of 27.12 kDa. It migRates as an approximately 48.86 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens ]
• Official Symbol: CD80
• Synonyms: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681