Acitve Recombinant Human BIR2 & BIR3 protein(Glu144-Leu356), Avi-tagged

• Cat.No.: BIR2 & BIR3-1236H
• Product Overview: Recombinant BIR2 & BIR3 domains (Glu 144-Leu 356) of Human cIAP1 (NP_001157.1) was expressed in E. coli, fused with the AVI tag at the C-terminus, and two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Species: Human
• Source: E.coli
• Tag: Avi
• ProteinLength: Glu144-Leu356
• Form: Lyophilized from sterile 10mM Tris, 5% glycerol, 0.5mM EDTA, 5mM DTT, pH 7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC50 for this effect is typically 25-750 nM.
• Molecular Mass: The recombinant Human cIAP1 consists of 230 amino acids and has a calculated molecular mass of 26.5 kDa as estimated by SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: BIR2; BIR3