Acitve Recombinant Canine NTRK1 protein(Pro285-Lys410), hFc-tagged

• Cat.No.: NTRK1-1322C
• Product Overview: Recombinant Canine NTRK1 (XP_851619.3) (Pro285-Lys410) was expressed in HEK293 with the Fc region of human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Canine
• Tag: Fc
• Protein length: Pro285-Lys410
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit NGF-induced prolifeRation of TF?1 Human erythroleukemic cells.The ED50 for this effect is typically 2-8 μg/mL in the presence of 10 ng/mL of recombinant Human NGF.
• Molecular Mass: The recombinant Canine NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 386 amino acids and has a predicted molecular mass of 42.3 kDa. The apparent molecular mass of the protein is approximately 55-61 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: NTRK1