Acitve Recombinant Rat NTRK1 protein(Met1-Pro418), hFc-tagged

• Cat.No.: NTRK1-1352R
• Product Overview: Recombinant Rat NTRK1 (P35739-Isoform TrkA-II)(Met1-Pro418) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: Met1-Pro418
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit NGF-induced prolifeRation of TF?1 Human erythroleukemic cells.The ED50 for this effect is typically 0.01-0.1 μg/mL in the presence of 10 ng/mL of recombinant Mouse NGF.
• Molecular Mass: The recombinant Rat NTRK1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 627 amino acids and has a predicted molecular mass of 69.2 kDa. The apparent molecular mass of the protein is approximately 92-102 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ntrk1 neurotrophic tyrosine kinase, receptor, type 1 [ Rattus norvegicus ]
• Official Symbol: NTRK1
• Synonyms: NTRK1; neurotrophic tyrosine kinase, receptor, type 1; high affinity nerve growth factor receptor; trk; trk-A; p140-TrkA; TrkA neurotrophin receptor; trkA proto-oncogene receptor; tropomyosin-related kinase A; slow nerve growth factor receptor; Trk
• Gene ID: 59109
• mRNA Refseq: NM_021589
• Protein Refseq: NP_067600