V5 CoIP Agarose kit

• Cat.No.: V5-03A
• Product Overview: The V5-Trap Agarose Kit contains V5-Trap Agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of V5-tagged proteins and their interacting factors.
  • Fast, reliable & efficient one-stepImmunoprecipitation
  • Effective elution with V5-peptide
  • No heavy & light antibody chains on the SDS-PAGE
  • Stable under harsh washing conditions
  • Suitable for downstream mass spec analysis

Specification

• Specificity/Target: V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
• Binding capacity: 15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: V5-peptide
  SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 40 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP, Protein purification