V5 CoIP Agarose

• Cat.No.: V5-01A
• Product Overview: Immunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to beads.
  • No heavy & light antibody chains in downstream applications
  • Very low background
  • Effective elution with V5-peptide
  • Stable under harsh washing conditions
  • EfficientImmunoprecipitation of V5-tag fusion proteins

Specification

• Specificity/Target: V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
• Binding capacity: 15 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: V5-peptide
  SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 40 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP, Protein purification
• Storage: Upon receipt store at +4°C. Do not freeze!