ULK1 Kinase Assay Kit

• Cat.No.: Kit-2535
• Product Overview: ULK1 (unc-51 like autophagy activating kinase 1) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. Conserved C-terminal domain of ULK1 controls the function and the localization of the protein. Knockdown of ULK1 inhibits the autophagic response and prevents rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of the ULK1 protein. ULK1 is phosphorylated upon activation of the mTOR pathway in a nutrient starvation-regulated manner. Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson’s disease.
  The ULK1 Kinase Assay Kit comes in a convenient 96-well format, with enough purified ULK1, MBP substrate, ATP, and kinase assay buffer for 96 enzyme reactions.

Specification

• Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput (HTS)
  applications
• Storage: Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Shipping: Dry ice
• Kit Components: ULK1: 20 µg, -80°C;
  Kinase Buffer 1 (5X): 1.5mL, -20°C;
  ATP (500 µM): 100µL, -20°C;
  Myelin basic protein (MBP), 5 mg/ml: 200µL, -20°C;
  96-well plate, white: 1, Room Temp.
• Materials Required but Not Supplied: Kinase-Glo MAX
  Microplate reader capable of reading luminescence
  Adjustable micropipettor and sterile tips
  30°C incubator