UDP Glycosyltransferase Assay kit

• Cat.No.: KITZ-050
• Product Overview: The UDP Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product. Glycosylation reactions catalyzed by glycosyltransferases are central to many biological processes, including cell:cell interactions, cell signaling and bacterial cell wall biosynthesis.
  This assay detects only the activity of glycosyltransferases that use UDP-sugar as a donor substrate and can only be used with purified glycosyltransferases not whole cells or cell extract. However, glycosyltransferases can be purified from cell extract using immunoprecipitation or affinity tag pull down then used in the assay.

Specification

• Size: 200 Assays
• Storage: Store the UDP Glycosyltransferase Assay at less than –65°C or store UDP Enzyme at less than –65°C and the other components at –20°C.
• Shipping: Dry ice
• Kit Components: 10mM UDP: 100 μl
  UDP Enzyme: 20 μl
  Enzyme Dilution Buffer: 1.5ml
  Nucleotide Detection Buffer: 5ml
  ATP Detection Substrate (lyophilized): 1 vial
• Materials Required but Not Supplied: Solid white multiwell plate (do not use black plates or clear plates)
  Enzyme reaction buffers; used for enzyme, substrate and compound dilution
  Multichannel pipette or automated pipetting station
  Glycosyltransferase
  Sugar acceptor substrate
  Luminometer capable of reading multiwell plates
  Plate shaker