TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

• Cat.No.: Kit-0904

Specification

• Tag: Non
• Description: The TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.
  To assay:
  When running the TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound’s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer. This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only " format.
• Applications: Competitive Binding Assay
• Storage: Store in ultra-cold freezer (-68 to -85°C).
• Shipping: Dry Ice
• Size: 800 x 20 µL assays
• Materials Required but Not Supplied: Microplate Reader
• Detection method: Fluorescent