TR-FRET LXR beta Coactivator Assay Kit, rabbit

• Cat.No.: Kit-0968

Specification

• Description: The TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
  How it works:
  In theTR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide.
• Applications: Nuclear Receptor Assay
• Storage: Tb-anti-GST Antibody (Rabbit): Store at -20 °C
  LXR beta-LBD, GST: Store at -80 °C
  Fluoroscein Coactivator Peptide: Store at -20 °C
  TR-FRET Coregulatore Buffer i: Store at 4 °C
  1M DTT: Store at -20 °C or -80 °C
• Size: 800 x 20 µL assays
• Kit Components: The TR-FRET LXR beta Coactivator Assay Kitcontains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
• Materials Required but Not Supplied: Microplate Reader
• Detection method: Fluorescent
• Tag: Non