TR-FRET ER beta Competitive Binding Kit

• Cat.No.: Kit-0932

Specification

• Description: The TR-FRET ER beta Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) beta. The kit uses a human ER beta ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (ES2) in a homogeneous mix-and-read assay format.
  The Lanthascreen ER beta Competitive Binding Assay is:
  • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
  • High throughput compatible—TR-FRET assays in a 384-well plate
  • Convenient—components available in bulk sizes for larger screening needs
• Applications: Competitive Binding Assay, Nuclear Receptor Assay
• Storage: 1 tube ER beta Ligand Binding Domain Recombinant Protein (store at -68 to -85°C)
  1 tube of Fluormone ES2 (store at -5 to -30°C)
  2 bottles NR Buffer (store at -5 to -30°C)
  1 tube Tb-Anti-GST Ab (store at -5 to -30°C)
  1 tube of DTT (store at -5 to -30°C)
• Size: 800 x 20 µL assays
• Tag: Non