Total Antioxidant Status Assay Kit

• Cat.No.: kit-2174
• Product Overview: Antioxidants in the sample reduce dark blue-green colored ABTS radical to colorless reduced ABTS form. The change of absorbance at 660 nm is related with total antioxidant level of the sample. The assay is calibrated with a stable antioxidant standard solution which is traditionally named as Trolox Equivalent that is a vitamin E analog.

Specification

• Storage: 4°C
• Kit Components: All reagents and standards are ready to use.
  Reagent 1 (Assay Buffer) 1 x 50 ml
  Reagent 2 (Colored ABTS Radical Solution) 1 x 10 ml
  *Standard 1 (0.0 mmolTrolox Equiv./L) Solution(Not included)
  Standard 2 (1.0 mmolTrolox Equiv./L) Solution1 x 10 ml
  *You should use any deionised-water
• Materials Required but Not Supplied: A spectrophotometer or a plate reader or an automated biochemistry analyzer.
• Compatible Sample Types: Blood serum, plasma, semen plasma, saliva, urine, cell lysates, tissue homogenates, beverages, fruit juices and oils (oils require different reagent 1) can be used as sample.
• Assay Protocol: 1. Manual Study
  Place 500 microliter Reagent 1 in cell and add 30 microliter standard (or sample). Read the initial absorbance at 660 nm for the first absorbance point.
  Add 75 microliter Reagent 2 to the cell and incubate 10 min at room temperature or 5 min at 37oC. Read the absorbance a second time at 660 nm.
  Calculating the Results
  Result = [ (∆Abs Std1) - (∆Abs Sample) ]/[ (∆Abs Std1) - (∆Abs Std2) ]
  ∆ Absorbance Standard1= (Second Absorbance of Std1- First Absorbance of Std1)
  ∆ Absorbance Standard2 = (Second Absorbance of Std2- First Absorbance of Std2)
  ∆ Sample Absorbance = (Second Absorbance of Sample- First Absorbance of Sample)
  2. Automated measurement is performed as same procedure. Only incubation time is shortened from 10 min to 5 min. Other parameters are similar. The volumes of reagents and sample are reduced at same ratio.
• Tag: Non