Thiopropyl Agarose

• Cat.No.: Thio-001A
• Product Overview: Thiopropyl Sepharos contains reactive 2-thiopyridyl disulphide groups attached to agarose through a chemically stable ether linkage, which reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for reversible immobilization of thiol containing molecules.

Specification

• Cat.no: Thio-001A
• Characteristics: 1. separate thiol-containing proteins and peptides from non-thiol-containing proteins and peptides2. in certain cases separate enzymes with active-site thiol groups from denatured enzymes3. store and protect thiol-containing proteins4. regenerate both the coupled biomolecule and the active bed material
• Size: 15 g
• Matrix: 6% agarose
• Functional group: 2-pyridyl disulphide
• Ligand concentration: 18–31 μmole 2-pyridyl disulphide/ml drained medium
• Coupling chemistry: approx 10–20 mg protein/ml drained medium, e.g. 14 mg ceruloplasmin (Mr 132 000) /ml drained medium
• Recommended column height: 5‐20cm
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Azides should be avoided.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 2-8
• pH CIP range: 2-8
• Tag: Non
• Publications:
  The role of an amphiphilic helix and transmembrane region in the efficient acylation of the M2 protein from influenza virus (2023)
  The role of an amphiphilic helix and transmembrane region in the efficient acylation of the M2 protein from influenza virus (2023)