TEV Protease Fluorometric Activity Assay Kit

• Cat.No.: Kit-2138
• Product Overview: Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins. It recognizes specific sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Glutamine and Glycine residues. TEV protease is active even at 4°C, making it the protease of choice for fusion proteins or tags removal. TEV Protease Assay Kit uses TEV specific Glu-Asn-Leu-Tyr-Phe-Gln-Gly substrate labeled with 5-FAM dye and quencher. Proteolytic cleavage of this quenched 5-FAM-quencher conjugate yields a brightly green fluorescence, which can be continuously monitored at Ex/Em= 490/520 nm. Increase in fluorescence intensity is directly proportional to the TEV protease quantity/activity.

Specification

• Materials Required but Not Supplied: 96-well microplate: Black, flat bottom 96-well plate with non-binding surface. Fluorescence microplate reader: Capable of excitation at 490 nm and detecting emission at 520 nm.
• Kit Components: Component A: TEV Substrate, Ex/Em=490 nm/520 nm upon cleavage: 50 µL Component B: 5-FAM, Fluorescence Reference Standard, Ex/Em=490 nm/520 nm: 100 µM, 10 µL Component C: TEV Protease, Recombinant: 2 µG X 4 vials Component D: Assay buffer: 30 mL Component E: DTT, 1M: 30 µL
• Storage: Store all kit components at -20℃, except for Component C. Store Component C at -80℃.
• Size: 100 Assays