TAF1L (BD1/BD2) Inhibitor Screening Assay Kit

• Cat.No.: Kit-1874

Specification

• Size: 384 reactions
• Description: The TAF1L (BD1 + BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1L binding to its substrate. The key to the assay kit is specific binding of the TAF1L bromodomains 1 and 2 (a.a. 1398-1649) to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1L and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
• Applications: Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
• Storage: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Kit Components: GST-TAF1L (1398-1649): 20 µg; -80°CBET Bromodomain Ligand: 400 µl; -80°C Non-acetylated Ligand 1: 200 µl; -80°C 3x BRD Homogeneous Assay Buffer 2: 4 ml; -20°C3x BRD Homogeneous Detection Buffer 2: 3 ml; -20°C
• Tag: Non