T cell Activation Beads

• Cat.No.: T-3015M

Specification

• Species: Human
• Beads Size: 500 μL
• Capacity: Up to 1×10^8 T cells
• Formulation: Biodegradable matrix-coated nanoparticles conjugated with anti-CD3 and anti-CD28 antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum Albumin (HSA), pH 7.0 - 7.4.
• Background: The T cell Activation reagent induces T cell activation and expansion in enriched T cells or PBMCs. The T cell Activation reagent is composed of biodegradable matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse anti-human CD28 antibodies. The small sized particulate structure of the activation reagent enables its easy removal by media washing or centrifugation.
  The recommended titer of 1:200 has been found to achieve efficient stimulation of majority of T cell subsets. For special applications, the optimal stimulation titer should be determined experimentally. Excessive amount of the T cell Activation reagent may result in over-activation of T cells risking activation-induced cell death. During cell expansion using the T cell Activation reagent, the cells take up to 13 days after reagent application to show optimal expansion. Restimulation after 13 days can be performed for longer cultivation. The T cell Activation reagent can be used together with cytokines such as human IL-2, IL-15, and IL-7 for more efficient activation.
• Application: T cell Activation reagent has been developed for in vitro activation and expansion of enriched T cell populations or cells from hematological cell populations (e.g. PBMCs).
• Storage: Store at 2-8 °C. Do not freeze.