Spot Magnetic CoIP Agarose

• Cat.No.: Spot-01A
• Product Overview: Immunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to Magnetic Agarose.
  • Magnetic separation with easy and efficient washing of Spot-Trap Magnetic Agarose
  • IP and Co-IP of Spot-tagged proteins without size limitation
  • No contaminating heavy and light antibody chains
  • Suitable for downstream mass spec analysis
  • Recombinantly expressed without batch-to-batch variation
  • Well-characterized and validated
  • Ready-to-use
  
  • Short incubation time of 30-60 minutes

Specification

• Specificity/Target: Spot-Tag sequence PDRVRAVSHWSS
• Binding capacity: 17.5 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 μm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer
  10 mM NaOH pH 12
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.1 % SDS
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 6 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP
• Storage: Upon receipt store at +4°C. Do not freeze!