Spot CoIP Agarose

• Cat.No.: Spot-03A
• Product Overview: Immunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to agarose beads.
  • No contaminating heavy and light antibody chains
  • Ready-to-use
  • Recombinantly expressed without batch-to-batch variation
  • Well-characterized and validated
  • High stability allows for stringent washing
  • Extraordinary low background compared to other affinity resins
  • Short incubation time of 30-60 minutes

Specification

• Specificity/Target: Spot-Tag sequence PDRVRAVSHWSS
• Binding capacity: 17.5 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  10 mM NaOH pH 12
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.1 % SDS
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 6 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP