SPHK1 Assay Kit

• Cat.No.: KITZ-029
• Product Overview: SPHK1 is a lipid enzyme that catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP). It has been implicated in tumor progression through cell proliferation and motility. High levels of SPHK1 have been associated with increased mortality in various forms of human cancer. SPHK1 Assay Kit is designed to measure SPHK1 activity for screening and profiling applications using Kinase-Glo MAX as a detection reagent. The SPHK1 Assay Kit comes in a convenient 96-well format, with enough purified SPHK1, Sphingosine, ATP, and kinase assay buffer for 96 enzyme reactions.

Specification

• Size: 96 reactions
• Storage: -80°C
• Shipping: Dry ice
• Kit Components: SPHK1, His-tag: >1 μg; -80°C.
  5x Kinase assay buffer: 1.5 ml; -20°C.
  ATP (500 μM): 100 μl; -20°C.
  Sphingosine (1 mM): 100 μl; -20°C.
  96-well plate, white: 1; RT.
• Materials Required but Not Supplied: Kinase-Glo MAX
  Dithiothreitol (DTT, 0.5 M)
  Microplate reader capable of reading luminescence
  Adjustable micropipettor and sterile tips
  30°C incubator