SNAP/CLIP-tag CoIP Agarose beads, kit

• Cat.No.: SNAP/CLIP-tag-02A
• Product Overview: The SNAP/CLIP-tag-Trap Agarose Kit contains SNAP/CLIP-tag-Trap Agarose, lysis, wash, and elution buffers for efficientImmunoprecipitation of SNAP-tag and CLIP-tag fusion proteins and their interacting factors.
  • SNAP/CLIP-tag-Trap immunoprecipitates SNAP-tag- and Clip-tag- fusion proteinseven when already covalently bound to ligands such as dyes, biotin etc.
  • Bound SNAP- or CLIP-tag fusion proteins can be eluted without protease
  • SNAP/CLIP-tag-Trap can be used for affinity purification
  • Structure and function are characterized

Specification

• Specificity/Target: SNAP-tag, CLIP-tag
• Binding capacity: 7.5-10 μg of recombinant SNAP/CLIP-tag per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 μm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer
  0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Affinity (KD): Dissociation constant KD of 1 nM
• Storage Buffer: 20% ethanol
• Applications: IP, CoIP, ChIP, RIP